Different from the 66KDa ERα (ERα66), high ERα36 expression was observed on the plasma membrane and cytoplasm of renal cancer specimens.24,35 As a truncated isoform of ERα66, ERα36 gene completely matches with exon2 to exon6 of ERα66 gene.19,36 Some epitopes are shared by ERα36 and ERα66 proteins, which explain the cytoplasm pattern of ERα66 expression that was observed in renal carcinoma tissues.15 Here, the specific antibody for ERα36 was generated from the unique peptide in ERα36-C terminal. This evidence concerns the gene ESR1 and renal carcinoma.