In AD patients, two-way ANOVA analysis indicated a significant negative effect of older age, independent of the presence of the ApoE4 allele, on the levels of amyloid deposition in diffuse cortical and subcortical ROIs: CCTXR (F1,128 = 4.47, p = 0.036), frontal (F1,128 = 4.29, p = 0.040), parietal cortex (F1,128 = 4.89, p = 0.029), anterior cingulate (F1,128 = 4.96, p = 0.028), posterior cingulate (F1,128 = 4.28, p = 0.041), insular cortex (F1,128 = 8.20, p = 0.005), thalamus (F1,128 = 4.71, p = 0.032) and hippocampus (F1,128 = 8.46, p = 0.004). This evidence concerns the gene APOE and Alzheimer disease.