Similar results were obtained when we analyzed the β-catenin/TCF/LEF pathway in DLD1 colon cancer cells stably expressing Daple-WT or FA mutant (Figure 5—figure supplement 1C) using 7-TGP, an eGFP expressing Wnt activity reporter construct (Fuerer and Nusse, 2010). This evidence concerns the gene HNF4A and malignant colon neoplasm.