To investigate the potential interaction between Nutlin-3 and CDDP in the p53 wild type NSCLC cell line A549, tumor cells were incubated with 0-20 μM CDDP combined with either simultaneous or sequential treatment of 0 μM, 5 μM, 10 μM or 25 μM Nutlin-3 for 24 hours. This evidence concerns the gene TP53 and non-small cell lung carcinoma.