In addition, using the same technique, we found that FAM83D was highly expressed in the HCC cell lines SMMC7721, PLC, MHCC97H, QGY7701, QGY7703, Huh7, HepG2, BEL7402, BEL7404 and BEL7405, whereas there was weak expression in MHCC-LM6, MHCC-LM3, MHCC97L and Hep3B cells. Here, SACK1D is linked to hepatocellular carcinoma.