To address this, we consequently knocked down Madcam1 and AKT, followed by Wortmannin treatment in Bel-7402 cells, and found that the p-AKT level was gradually decreased (Figure 7H-7I) and accompanied by gradually decreased Doxo-induced Caspase 3/7 activities (Figure 7J), suggesting that a reduction of p-AKT may decrease Doxo sensitivity in HCC cells. This evidence concerns the gene CASP3 and hepatocellular carcinoma.