Interestingly, as well as bvPTX3, our preliminary results showed that the recombinant PTX3 protein purified from mammal cells (euPTX3) has no effect on the proliferation of various cancer cells, but in agreement with the above observations, euPTX3 promoted sphere-forming ability in vitro, attenuated E-cadherin and increased N-cadherin, Twist1 and Snail2 transcripts, increased drug-resistance and migration and invasion of mcCDRMB231 cells in vitro. The gene discussed is TWIST1; the disease is cancer.