Since our previous data, showing significant effects of DIAPH1 on the metastatic potential of colon cancer metastasis in vitro and in vivo, were not based on LPA stimulation, one main goal of this study was to analyze DIAPH1-mediated cellular effects on non-stimulated HCT-116 colon cancer cells more closely. Here, DIAPH1 is linked to malignant colon neoplasm.