Although exclusion of CD127 only led to a substantial increase in the frequency of def.1 Tregs (mean 21.5 %, range 14.7–29.3 %) in the PBMC of OvCa patients, exclusion of CD25 or CD25 and CD127 led to substantial increases in the frequency of these def.1 Tregs in PBMC of HD and OvCa patients as well as in TDLN or tumor samples from CxCa patients (17.9, 21.9, 24.0, and 30.8 % for CD25 exclusion and 37.6, 58.8, 40.4, and 43.3 % for CD25 and CD127 exclusions, see supplementary figure 10a). Here, IL7R is linked to neoplasm.