As shown in Fig. 3 for representative examples, the gating and enumeration of Tregs based on CD25, CD127, Foxp3 (def.1), and Foxp3 and Helios (def.2) is feasible in OvCa-derived peripheral blood, as well as in TDLN and tumor samples from CxCa patients using the same gating strategy applied for HD-derived PBMC. This evidence concerns the gene IKZF2 and neoplasm.