FOXP3 and neoplasm: Treg enumeration based on def.3 was feasible in peripheral blood and TDLN samples of patients but was not reliable in tumor samples due to the absence of the Foxp3intCD45RApos nTreg population which is used for discrimination between Foxp3int and Foxp3hi cells in the gating strategy (see also Supplementary figure 5 for Foxp3 and CD45RA gating strategy).