S1 Material showed the part of detailed information of the patients including their FNA-Tg and TgAb levels. Table 1 showed the clinical characteristics and US features of the LNs without and with metastasis. On univariate analysis, LNMs were significantly associated with male gender, larger primary tumor size, presence of lymph node metastasis at the initial operation, and higher serum Tg levels (Table 1). This evidence concerns the gene TG and metastatic malignant neoplasm in the lymph nodes.