28 CpG loci including APC, CACNA1G, CHFR, COX2, DAPK, DCC, HPP1, MGMT-Mp region, MGMT-Eh region, MINT1, MINT2, MINT31, MLH1 5', MLH1 3', p14, p16, RASSF1A, RASSF2A-region1, RASSF2A-region2, RASSF3, RASSF5, RASSF6, RUNX3, SFRP2-region1, SFRP2-region2, UNC5C, 3OST2 and FOXL2 were analyzed for determining the CIMP status of each gastric cancer. The gene discussed is MLH1; the disease is gastric cancer.