HIF1A and hepatocellular carcinoma: We further evaluated the effect of rs2057482 genotypes on mRNA expression of HIF1A in 40 HCC tissues (20 with CC genotype and 20 with CT/TT genotypes) using qRT-PCR and found that mRNA expression level of HIF1A was significantly lower in patients carrying CT/TT genotypes of rs2057482 than those in patients carrying CC genotype (P = 0.014) (Fig. 2D).