Moreover, treatment of DU145 and PC-3 mPGES-1SC cells with mPGES-1 inhibitor MF63 (10 μmol/l) and DU145 mPGES1SC cells with COX-2 inhibitor NS398 (10 μmol/l, 96 h) inhibited EGFR expression (Fig. 6E and F), while exogenous PGE2 (1 μmol/l, 96 h) increased EGFR protein levels in DU145 mPGES-1KD cells, supporting involvement of PGE2 in promoting EGFR over-expression in prostate tumours. This evidence concerns the gene PTGS2 and prostate neoplasm.