To study the immune-modulatory activities of ebselen in a topical application against MRSA skin infection, we used ELISA to measure the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) and monocyte chemo attractant protein-1(MCP-1) in the infected wounds. This evidence concerns the gene TNF and methicillin-resistant staphylococcus aureus infectious disease.