The aims of the present study include: i) the application of the FCBA in a clinical laboratory setting for the rapid diagnosis of CML in order to evaluate specificity and selectivity of BCR-ABL1 detection in comparison with routine RQ-PCR testing; ii) evaluation of the potential use of FCBA in the follow-up of patients treated with TKIs and monitored in parallel with routine RQ-PCR testing; and iii) comparison of the sensitivity and specificity of FCBA and FISH in the clinical setting. Here, BCR is linked to chronic myelogenous leukemia, BCR-ABL1 positive.