CXCR4 and neoplasm: We also confirmed the accumulation of ascites in another highly peritoneal-metastatic subline 58As9 (derived from HSC-58) which expressed CXCR7 at a high level but not CXCR4. One exceptional case was 44As3 derived from HSC44 that had multiple tumor nodules and accumulated ascites without the expression of both CXCR4 and CXCR7. Overall, we found a high correlation between the expression level of CXCR4 or CXCR7 and the aggressive phenotypes in seven of the eight cell lines except 44As3.