NSG mice injected with 0.5x106 GFP-FFLuc NALM/6 cells, a pre-B-cell ALL cell line negative for T costimulatory ligands, developed diffuse leukemia mainly homed in liver and bone marrow, as illustrated by bioluminescence imaging in Fig 2A and confirmed by FACS detection of CD19+ cells (data not shown). Here, CD19 is linked to acute lymphoblastic leukemia.