We confirmed increased expression of KLF4 mRNA in carfilzomib-resistant MM cells by qRT-PCR analysis (Figure 2A), which was paralleled by a corresponding increase in KLF4 protein levels (~3.0 ± 0.7-fold, n = 4, P < 0.009 by paired Student's t test) detected by western blotting (Figure 2B). This evidence concerns the gene KLF4 and Miyoshi myopathy.