In our study, we found the up-regulation of Her-2, EGFR, P-EGFR and survivin proteins, along with a decrease of apoptosis in NCI-N87/TR cells, indicating that co-overexpression of Her-2 and EGFR might participate in regulating the resistance of gastric cancer to trastuzumab through up-regulating the expression of survivin protein. This evidence concerns the gene BIRC5 and gastric cancer.