The combined methodology applied herein not only confirmed the relevance and the involvement of the molecular triad, NPM1, GRP78 and RKIP with glioma malignancy, but additionally has uncovered two new important targets: NOVA1, useful for the diagnostic refinement between astocytoma and oligodendroglioma, and HSPB1, as a predictive factor of poor prognosis for glioblastoma. The gene discussed is HSPA5; the disease is central nervous system cancer.