Conversely, using the human CD4 T-cell line CEM-GXR25 that expresses GFP driven by HIV-1 LTR in a Tat-dependent manner25, we confirmed that constitutive inhibition of endogenous miR-29 by lentivirus-encoded antisense ‘miR-29-ZIP' enhanced infection with CXCR4-tropic HIV-1NL4-3 (Supplementary Fig. 5b). This evidence concerns the gene CXCR4 and infection.