CD69 and neoplasm: More importantly, PMA/Ionomycin stimulation of tumor suspensions revealed that IFN-α2 and IL-2 –treated tumors contained higher number of IFN-γ+ CD69+ CD8+ TILs compared to either control groups (Fig 5C), suggesting either increased tumor-infiltration of activated CD8+ T-cells or in situ activation of TILs following cytokine treatment.