To assess the degree of tumor-antigen specific T-cell infiltration in tumors as a possible explanation for the observed anti-tumor effects with the cytokine combinations, total CD8+ T-cells specific for MHC-I-loaded chicken ovalbumin SIINFEKL peptide (endogenous + transferred OT-I cells) were quantified by flow cytometry on days 4 and 14 post-transfer. Here, CD8A is linked to neoplasm.