Following our previous study demonstrating that HMGB1 increases miR221 and miR222 expression in short term primary cultures of papillary cancer cells compared with normal cells obtained from contralateral thyroid lobe and with cells from patients with nonneoplastic lesions, we report here that HMGB1 induces miRNAs 221 and 222 hyperexpression in both differentiated (BCPAP) and anaplastic (CAL62) thyroid cancer cell lines. Here, HMGB1 is linked to thyroid gland carcinoma.