To validate the findings in the integrated analysis, we selected 5 genes (COL4A2, BMF, DUSP1, FOXA1, MLPH) of the top 10 up or down regulated genes to perform qRT-PCR in seven human breast cancer cell lines including three TNBC lines (MDA-MB-231, MDA-MB-435, MDA-MB-468) and four non-TNBC lines (MDA-MB-453, MCF-7, BT-474, SK-BR-3). The gene discussed is COL4A2; the disease is breast cancer.