Based on MRS measurement of liver fat in a group of subjects considered to be at low-risk for NAFLD (i.e., subjects of normal weight, with normal fasting serum glucose and alanine aminotransferase concentrations, and without diabetes mellitus), the upper “normal” limit of IHTG has been defined as 5.6% of liver volume, which represented the 95th percentile in this reference population [10]. This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.