As shown in Figure 2A and in Supplementary Figure 1A, C4-27z CAR T cells produced significant amounts of IFN-γ and IL-2 after coculture with all αFRpos cancer cell lines, but not when cultured with αFRneg cells, indicating that C4 CAR T cells functionally recognize αFRpos tumor cells. The gene discussed is IFNG; the disease is cancer.