The results of this study showed that the expression levels of EGFR mRNA in ESCC was higher than that of adjacent tissues to esophageal carcinoma, and in the clinical and pathological features, the EGFR expression level was independent of gender, ethnicity, age, tumor differentiation, and clinical staging of patients with ESCC patients, but it was related with lymph node metastasis (P < 0.05). This evidence concerns the gene EGFR and metastatic malignant neoplasm in the lymph nodes.