In experiments to further help determine whether HMGB1 regulates VEGF-A in tumour angiogenesis, we applied the associated antibodies to co-cultured ECs, and VEGF-A concentrations were decreased by 2G7 mAb, TLR4 Ab and RAGE Ab, as demonstrated in Western blot analyses (Fig.3A). The gene discussed is TLR4; the disease is neoplasm.