Curious to know whether 21α-MMD can exert anti-oxidative function or in other way support the redox activity against NSCLC cells, we examined its activity on ROS-triggered regulatory utility by which 21α-MMD significantly increased intracellular ROS generation which might be related to Akt signal since an enhanced inhibition was observed after lung cancer cells were treated with relative low-concentration of 21α-MMD and N-acetyl cysteine, a radical scavenger. This evidence concerns the gene AKT1 and lung cancer.