However, significantly high level of hypermethylation was observed in p16, DAPK, ECAD, RASSF1, MINT1, MINT2 and MINT31 (P = 0.02, 0.02, 0.04, 0.02, 0.01, <0.01, and 0.01 respectively) in HNSCC tissues compared to normal tissue samples. The gene discussed is DAPK1; the disease is head and neck squamous cell carcinoma.