Englerin A sensitive A-498 renal cell carcinoma cells were transfected with TRPC4 siRNAs to determine whether TRPC4 mRNA knockdown affects the cell’s response to englerin A. Two different siRNAs reduced TRPC4 mRNA levels by over 80% relative to a non-targeting control siRNA (Fig 4A). The gene discussed is TRPC4; the disease is hereditary clear cell renal cell carcinoma.