In CRC cases with prominent CSC-like transition zones, we examined the genomic findings by microarray (Table 2) and the mutational findings by sequencing panels, including for the exon 3 mutation hotspot in CTNNB1 as well as KRAS, PTEN, SMAD4, and TP53. Analysis was performed in macrodissected tumor within the invasive areas. The gene discussed is SMAD4; the disease is colorectal carcinoma.