To verify that tumor regression specifically requires PI3K/Akt activation in the stroma, we evaluated the response of these treatments on C4-HD tumor cells growing on 3D Matrigel (Supplementary Figure S3A), finding again that MFP was the most effective treatment for decreasing Ki67 and increasing caspase 3 staining (Supplementary Figure S3B, S3C). This evidence concerns the gene PIK3CA and neoplasm.