To further investigate if the decrease in cell growth observed after nanovesicles treatment was due to the activation of TRAIL-stimulated apoptotic cell death, Annexin V–FITC fluorescence was measured by flow cytometry on cancer cell lines treated for 48 h with 20 μg/ml of lemon nanovesicles in presence or absence of TRAIL neutralizing antibodies. The gene discussed is ANXA5; the disease is cancer.