To investigate whether the mosaic mutations in the MCR of apc resemble also functionally the situation in mammals and induce neoplasia with a hyperactive Wnt/β-catenin signaling pathway, we injected the apc TALENs in embryos obtained from a transgenic X. tropicalis Wnt reporter line that we previously generated and characterized [23, 24]. The gene discussed is APC; the disease is neoplasm.