The transfer of biotinylated membrane proteins (Figure 3A, top panels) was influenced by two factors: (i) the activation status of the T cells (high transfer only occurred onto anti-CD3-prestimulated CAR T and NT T cells but not onto primary CD8+ T cells) and (ii) the expression of the tumor-specific CAR (much more biotinylated proteins were transferred onto AC133-CAR T cells than onto NT T cells). The gene discussed is CD8A; the disease is neoplasm.