The QMSP results were consistent with our bisulfite sequencing results; higher methylation values were detected in 8 cell lines (011, 012, 022, 028, 5637, HT1376, J82, and SCaBER), all of which had no S100A2 expression in RT-PCR or Western blotting analysis, whereas the head&neck cancer cell lines 013, 019, Fadu, KYSE30, KYSE410, and KYSE520, which express S100A2, were unmethylated in this promoter region. This evidence concerns the gene S100A2 and head and neck cancer.