We also assessed expression levels of the autophagy-related genes Atg7, Atg8a and Atg12. Quantitative real-time PCR (qRT-PCR) data from DM1 flies and controls [Fig. 3I; hs-Gal4 UAS-i(CTG)480>UAS-GFP compared to hs-Gal4>UAS-GFP UAS-GFP] revealed that there was a more than 2-fold increase in the expression of these genes. Here, ATG12 is linked to myotonic dystrophy type 1.