Zentilin et al. (2010) also found Serca2α, Ryr2 and Pgc1α to be significantly decreased following myocardial infarction, accompanied by hypertrophy and dysfunction. In our HART model, we found that Serca2α levels dropped only slightly, whereas Ryr2 showed a more substantial tendency to decrease (Fig. 5E, supplementary material Fig. S3C). Taken together with the tendency towards reducing levels of Vegfα driven mainly by the NM population, dysregulation of Ryr2 might play a part in initiation of CM dysfunction in our model. The gene discussed is PPARGC1A; the disease is myocardial infarction.