Here we show that KRS is required for dissemination of single or small-groups of cells from colon cancer spheroids in 3D collagen I gels, where E-cadherin and K14 were maintained at the cell-cell junctions; this suggests a new mode of KRS-dependent dissemination in cells that retain epithelial markers at the junctions. This evidence concerns the gene KARS1 and malignant colon neoplasm.