To examine the possible induction of CPT-11 resistance by fibroblast-derived HGF and the efficacy of combined treatment with anti-HGF antibody and CPT-11 on colorectal cancer in vivo, we generated xenografts by inoculating HCT116 human colorectal cancer cells, with or without fibroblast CCD-18co cells, into NOD/SCID mice subcutaneously. Here, HGF is linked to colorectal cancer.