Since we conduced most of our experiment to test the regulation of mir3686 to PLK1 in pancreas cancer cell line PANC1 and the immortalized pancreas cell HPDE6C7 cell demonstrated a stronger expression of mir3686, we performed the “gain of function” assay in HPDE6C7 cell to confirm our previous finding in another way. This evidence concerns the gene PLK1 and pancreatic neoplasm.