Thus, we think that the EAE may have downregulated MMP-9 protein expression and upregulated nm-23H1 in HCC in vivo; the more reduced the MMP-9 protein expression the less degraded the extracellular matrix; cancer cell migration and invasion were suppressed, the same effect as nm23-H1 increased in HCC. This evidence concerns the gene NME1 and hepatocellular carcinoma.