Staining of GLUT4 vesicles was observed as cytoplasmic aggregates in biopsies from patients 3, 5, and 7 (Figure 4), although in patients 3 and 7 there are a smaller number of these aggregates; the three patients presented hyperinsulinemia and IR (Table 3) and patient 5 showed hyperglycemia. This evidence concerns the gene SLC2A4 and hyperinsulinism.