We found that the basal PAI-1 activity levels were significantly higher in T2D patients with CAD (group A) compared to T2D patients without CAD (group B) (P < 0.01), as well as in nondiabetic patients with CAD (group C) compared to control group (group D) (P < 0.05). This evidence concerns the gene SERPINE1 and type 2 diabetes mellitus.