This study utilizes both the novel MALDI IMS technique and, where possible, traditional IHC to examine changes in A-series ganglioside expression in rats who underwent either surgical striatal endothelin-1 (ET-1) injection (stroke alone group), bilateral intracerebralventricular (icv) injections of beta-amyloid (Aβ25–35) (Aβ alone group), or ET-1 with bilateral icv injections Aβ25–35 (combined Aβ/ET-1 group). Here, EDN1 is linked to stroke disorder.