To evaluate the relation between POSTN expression and the ability to attract melanoma cells, we injected shControl or shPOSTN MC3T3-E1 cells mixed with shControl or shPOSTN MC3T3-E1 cell-conditioned medium, which was added for the purpose of introducing a peak POSTN expression similar to endogenous POSTN expression during the wound healing process, and matrigel into the thigh 7 days after injection of B16-BL6 melanoma cells into the footpad of nude mice (Fig 6A). The gene discussed is POSTN; the disease is melanoma.