EGFR and non-small cell lung carcinoma: As seen in the pyrosequencing traces of the targeted sequence of the six cell lines, the NSCLC cell lines H1650 (c.2235_2249del15, Fig. 1aiii) and H1975 (c.2573T>G, Fig. 1aiiii) showed specific peak patterns, which were readily distinguishable from that of the EGFR exons 19 and 21 wild-type NSCLC cell line A549 (Fig. 1ai and ii).