To examine the knockdown effect of Swiprosin-1 on metastasis of melanoma, shRNA targeting Swiprosin-1 regions conserved in both human and mouse (sh#1) or specific for either human (sh#2) or mouse (sh#3) sequences were designed, and their silencing efficiencies verified using RT-PCR and immunoblotting. The gene discussed is EFHD2; the disease is melanoma.