In conclusion, siRNA targeting of B7-H4 effectively suppressed the proliferation, invasion, and migration of LOVO cells by inhibiting the effect of CXCL12/CXCR4 and the phosphorylation of JAK2/STAT3 on increasing the metastatic potential of colorectal carcinoma. This evidence concerns the gene CXCR4 and colorectal carcinoma.